nbt饱和溶液怎么配置
1. 钥冮┈鏂浜钃濇硶娴嫔畾鐗涜娓呰泲锏界殑镙囧嗳镟茬嚎锛
镙囧嗳镟茬嚎鍒朵綔钬旇冮┈鏂浜钃濇硶娴嬭泲锏借川钖閲
涓銆 镙囧嗳镟茬嚎
涓鑸鐢ㄥ垎鍏夊厜搴︽硶娴嬬墿璐ㄧ殑钖閲忥纴鍏堣佸埗浣沧爣鍑嗘洸绾匡纴铹跺悗镙规嵁镙囧嗳镟茬嚎镆ュ嚭镓娴嬬墿璐ㄧ殑钖閲忋傚洜姝わ纴鍒朵綔镙囧嗳镟茬嚎鏄鐢熺墿妫娴嫔垎鏋愮殑涓椤瑰熀链鎶链銆
浜屻 铔嬬槠璐ㄥ惈閲忔祴瀹氭柟娉
1銆 鍑姘忓畾姘娉
2銆 鍙岀缉鑴叉硶
3銆 Folin-閰氲瘯鍓傛硶
4銆 绱澶栧惛鏀舵硶
5銆 钥冮┈鏂浜钃濇硶
涓夈 钥冮┈鏂浜钃濇硶娴嫔畾铔嬬槠璐ㄥ惈閲忊旀爣鍑嗘洸绾垮埗浣
锛堜竴锛夈佽瘯鍓傦细
1銆 钥冮┈鏂浜钃濊瘯鍓傦细
钥冮┈鏂浜钃浒钬250 100mg婧朵簬50ml 95%涔欓唶锛屽姞鍏100ml 85% H3PO4锛岄泹钂搁忔按绋閲婅呖1000ml,婊ょ焊杩囨护銆傛渶缁堣瘯鍓备腑钖0.01%锛圵/V锛夎冮┈鏂浜钃浒钬250,4.7%锛圵/V锛変箼阅囷纴8.5%锛圵/V锛塇3PO4銆
2銆 镙囧嗳铔嬬槠璐ㄦ憾娑诧细
绾镄勭墰琛娓呰铔嬬槠锛岄勫厛缁忓井閲忓嚡姘忓畾姘娉曟祴瀹氲泲锏芥爱钖閲忥纴镙规嵁鍏剁函搴﹀悓0.15mol/LNaCl閰嶅埗鎴100ug/ml铔嬬槠婧舵恫銆
锛堜簩锛夈佸櫒𨱒愶细
1銆722S鍨鍒嗗厜鍏夊害璁浣跨敤鍙婂师鐞嗭纸锛夈
2銆佺Щ娑茬′娇鐢锛堬级銆
锛堜笁锛夈佹爣鍑嗘洸绾垮埗浣滐细
璇旷＄紪鍙 0 1 2 3 4 5 6
100ug/ml镙囧嗳铔嬬槠锛坢l锛 0.0 0.1 0.2 0.3 0.4 0.5 0.6
0.15mol/L NaCl 锛坢l锛 1 0.9 0.8 0.7 0.6 0.5 0.4
钥冮┈鏂浜钃濊瘯鍓 锛坢l锛 5 5 5 5 5 5 5
鎽囧寑锛1h鍐呬互1鍙风′负绌虹槠瀵圭収,鍦595nm澶勬瘆镩
A595nm
1銆
2銆佷互A595nm涓虹旱鍧愭爣锛屾爣鍑呜泲锏藉惈閲忎负妯鍧愭爣锛埚叚涓镣逛负10ug銆20 ug銆30 ug銆40 ug銆50 ug銆60 ug锛夛纴鍦鍧愭爣杞涓婄粯鍒舵爣鍑嗘洸绾裤
1锛夈佸埄鐢ㄦ爣鍑嗘洸绾挎煡鍑锲炲綊鏂圭▼銆
2锛夈佺敤鍏寮忚＄畻锲炲綊鏂圭▼銆
3锛夈佹垨鐢╫rigin浣滃浘 锛屾祴鍑锲炲綊绾挎ф柟绋銆傚嵆A595nm=a脳X( )+6
涓鑸鐩稿叧绯绘暟搴旇繃0.999浠ヤ笂锛岃呖灏2涓9浠ヤ笂銆
4锛夈佺粯锲炬椂杩戜袱浣跨偣鍦ㄤ竴𨱒＄洿绾夸笂锛屽湪鐩寸嚎涓婄殑镣瑰簲璇ュ湪鐩寸嚎涓や晶銆
锛埚洓锛夈佽泲锏借川钖閲忕殑娴嫔畾锛
镙峰搧鍗虫墍娴嬭泲锏借川钖閲忔牱鍝侊纸钖閲忓簲澶勭悊鍦ㄦ墍娴嬭寖锲村唴锛夛纴渚濈収镎崭綔姝ラ1镎崭綔锛屾祴鍑烘牱鍝佺殑A595nm锛岀劧钖庡埄鐢ㄦ爣鍑嗘洸绾挎垨锲炲綊鏂圭▼姹傚嚭镙峰搧铔嬬槠璐ㄥ惈閲忋
涓鑸琚娴嬫牱鍝佺殑A595nm鍊煎湪0.1钬0.05涔嬮棿锛屾墍浠ヤ笂杩版牱鍝佸傛灉A595nm鍊煎お澶э纴鍙浠ョ█閲婂悗鍐嶆祴A595nm鍊硷纴铹跺悗鍐嶈＄畻銆
锛堜簲锛夈佹敞镒忎簨椤癸细
1銆 鐜荤拑浠鍣ㄨ佹礂娑ゅ共鍑銆
2銆 鍙栭噺瑕佸嗳纭銆
3銆 鐜荤拑浠鍣ㄨ佸共鐕ワ纴阆垮厤娓╁害鍙桦寲銆
4銆 瀵圭収锛氱敤琚娴嬬墿璐ㄤ互澶栫殑鐗╄川浣灭┖锏藉圭収銆
钻鍝佺殑閰嶅埗锛纾烽吀缂揿啿娑镄勯厤鍒讹级
涓銆佽嵂鍝佺殑閰嶅埗姝ラ
锛堜竴锛夈佸疄楠屽嗳澶囷细
1銆 鍑嗗囨墍闇镄勮嵂鍝佸拰鐜荤拑浠鍣ㄣ
2銆 娲楁钉銆傦纸镐庢牱娲楁钉绠楀共鍑锛燂级
锛堜簩锛夈佽＄畻锛
1銆佺栌鍒嗘瘆娴揿害璁＄畻锛
1)銆丢/V姣
渚嫔傞厤1% NaCl锛岀О1g NaCl婧朵簬100ml 姘淬
2锛夈乂/V姣旓细
渚嫔傞厤75%涔欓唶100ml锛75%脳100%=100%脳X, X=75ml銆傚彇75ml镞犳按涔欓唶锛屽姞25ml钂搁忔按銆
涔欓唶锛氢箼阅氾细涓欓叜=2锛1锛2閰500ml锛屽悇鍙200 ml锛100 ml锛200 ml娣峰悎銆
3锛塆/V姣旓细鐢ㄧ殑杈冨皯锛屽傝＄畻𨱔板垎涓镆愮嶅厓绱犲侳e镄勫惈閲忋
2銆鎽╁皵娴揿害璁＄畻锛氭敞锛氲嵂鍝佺殑鍒嗗瓙閲忎竴鑸鍦ㄦ爣绛句腑娉ㄦ槑銆
1锛夈0.1M鎴0.1mol/L NaCl閰100ml銆
M=璐ㄩ噺/浣撶Н锛圠锛夌О鍙朜aCl0.1脳0.1脳40=0.4g 鎽╁皵鏁=G锛坓锛/鎽╁皵璐ㄩ噺
2锛夈0.1mMNaCl閰100ml
mM=姣鎽╁皵鏁/浣撶Н锛圠锛 绉板彇NaCl0.1脳0.1脳40=0.4g
姣鎽╁皵鏁=G锛坢g锛/鎽╁皵璐ㄩ噺
3锛夈0.1uNaCl閰100ml
mM=寰鎽╁皵鏁/浣撶Н锛圠锛 绉板彇NaCl0.1脳0.1脳40=0.4mg
寰鎽╁皵鏁=G锛坲g锛/鎽╁皵璐ㄩ噺 绉板彇NaCl0.1脳0.1脳40=0.4ug
3銆 娣峰悎婧舵恫閰嶅埗镄勮＄畻锛
濡傞厤3uMEDTA锛2.25mM NBT浠ュ强60uM 婧舵恫100ml锛岀敤50mM纾烽吀缂揿啿娑查厤鍒躲
娉ㄦ剰锛1銆佸垎鍒镙囧畾浣撶Н璁＄畻
2銆佸垎鍒閰嶅埗鍐嶆贩钖堬纴浣嗘讳綋绉涓嶈兘 涓100ml
锛堜笁锛夈佹爣閲忥细
1銆 镙规嵁闇瑕侀夋嫨涓嶅悓閲忕▼镄勫ぉ骞虫牴鎹瑕佹眰铡讳笉钖岀簿搴︾殑娴嬮噺鍣锛屽傞噺绛掓垨绉绘恫绠°
2銆 鐢靛瓙鍒嗘瀽澶╁钩镄勪娇鐢ㄣ
锛埚洓锛夈佹憾瑙ｏ细
1銆 镙规嵁钻鍝侀厤缃瑕佹眰阃夋嫨婧跺墏銆傝捀棣忔按锛屽弻钂告按锛屾棤绂诲瓙姘寸瓑銆
2銆 鍙鑳界敤鐑ф澂婧惰В銆傛敞镒忓姞鍏ユ憾鍓傚彧鑳藉姞鍏ユ讳綋绉镄2/3宸﹀彸锛屽墿浣欐憾鍓傛礂娑ょ儳𨱒涓夋″乏鍙筹纴鐩村埌娲楁钉骞插噣銆
灏忓父璇嗭细钻鍝佹爣绛句腑涓鑸镙囱瘑链夎嵂鍝佺殑婧惰В镐ц兘鍜屽垎瀛愬纺锛屽彲镙规嵁鍒嗗瓙寮忓拰镓瀛︾殑甯歌瘑鍒ゆ柇钻鍝佺殑缁撴瀯鍜屾ц川鐗圭偣锛埚寘𨰾婧惰В镐ц川锛夈
濡傞吀纰变袱镐х墿璐ㄧ殑閰嶅埗锛圆A銆佽泲锏借川銆镙歌嫹閰绛夛级濡傛灉婧惰В镐ц兘涓嶅ソ鍙浠ョ敤绋閰告垨绋纰变绩杩涙憾瑙ｏ纴浣唒H搴斿湪琚瑕佹眰镄勮寖锲村唴銆
3銆 锷犵儹淇冭繘婧惰В锛屼絾娉ㄦ剰搴斿湪閰嶅埗镄勮寖锲村唴链夌殑钻鍝佽缮闇姘存憾锷犵儹杈冨ソ銆傚:閰0.1%镄勬稳绮夛纴姘磋曞姞鐑锛堟俯搴﹀湪80-90銆侰锛夛纴杩囬噺浼氱硦鍖栥
锛堜簲锛夈佸畾瀹癸细
1銆 鐢ㄥ归噺鐡跺畾瀹癸绂
2銆 鐢ㄧ幓镨冩掑紩娴佹垨鐢ㄥ皬婕忔枟锛
3銆 鐢ㄦ憾鍓傚姞鍏ュ埌鎺ヨ繎鍒诲害锛岀劧钖庣敤婊寸″姞鍏ュ埌鍒诲害銆傝佹眰鍒诲害涓庢恫浣揿嚬闱㈢浉鍒囦负姝锛堢溂镌涘彲瑙嗭级锛
4銆 涓娄笅绐戞礊瀹归噺鐡跺嚑娆★纴娣峰悎鍧囧寑鍗冲彲銆(娉ㄦ剰涓嶅啀瀹氩逛简锛岄槻姝㈡憾娑叉纺鎺夈)
锛埚叚锛夈佽呭叆璇曞墏鐡讹纴璐翠笂镙囩俱
镙囩惧簲娉ㄦ槑浠ヤ笅鍐呭癸细钻鍝佹禄搴︺佸悕绉般侀厤鍒朵汉銆侀厤鍒舵棩链熺瓑銆
锛堜竷锛夈佹竻鐞嗗疄楠屽満镓銆
浜屻佺７閰哥紦鍐叉恫镄勯厤鍒躲
锛堜竴锛夈侀厤鍒0.2mol/L鍗0.2M pH=6.8镄勭７閰哥紦鍐叉恫銆傜敤纾烽吀姘浜岄挔钬旂７閰镐簩姘㈤挔锅氱紦鍐叉恫銆
阃夌敤钻鍝侊细Na2HPO412H2O锛堢⒈锛夊拰NaH2PO412H2O锛堥吀锛夐厤缂揿啿娑100ml銆备功涓璇存槑銆
锛堜簩锛夈佽＄畻 锛
1銆 娉锛氢功涓链夋敞鏄庨厤缃镄勯噺銆
2銆 璁＄畻锛歂a2HPO412H2O绉板彇71.64脳0.1=7.164g
NaH2PO412H2O绉板彇 31.21脳0.1=3.121g
3銆 钖链変笉钖岀粨鏅舵按镄勬崲绠椼
涔︿腑閰1/15mol/L镄勭紦鍐叉恫閰1L锛屼功涓鐢∟a2HPO42H2O闇瑕11.87g/L浣嗙敤Na2HPO412H2O闇澶氩皯g锛
11.87=锛178/358锛壝梄锛孹=23.87g銆
锛堜笁锛夈佸垎鍒閰嶅埗缂揿啿娑插悇100ml锛堟牴鎹闇瑕佺‘瀹氶厤鍒剁殑閲忥级銆
鍗筹细0.2M Na2HPO42H2O鍜孨aH2PO42H2O100ml銆
锛埚洓锛夈佹寜涔︿腑镄勯噺閰嶅埗鍙栭噺鍐嶆贩钖堛
濡傦细pH=6.8锛屽垎鍒铡荤紦鍐插49ml鍜51ml銆
锛堜簲锛夈佺敤pH璇旷焊ceni绱㈣禂镄勭紦鍐叉恫镄pH鍊锛屾娴嬮厤缃鏄钖﹀嗳纭銆
锛埚叚锛夊傞厤50mM pH =6.8镄勭紦鍐叉恫100ml 锛屼互浣犻厤鍒剁殑姣嶆恫绋閲婂嵆鍙銆
璁＄畻锛50脳0.1=0.2脳1000脳X 锛圠锛夛绂X =0.025L锛
鍙0.2M pH=7.2镄勭紦鍐叉恫25ml锛岀敤钂搁忔按瀹氩硅呖100ml鍗冲彲銆